Two men in their 40s were killed when they were run over in a tunnel on Route 60 near the Har Gilo settlement in Gush Etzion on Friday morning.
They were found with multiple system injuries and paramedics pronounced them dead at the scene.
Police have identified them as construction workers who may be on their way to a nearby construction site.
Police said they have launched an investigation into the incident.
Route 60 runs from Beer Sheva in the south to Nazareth in the north, mostly passing through the West Bank.
Last month, a truck collided with a car on Highway 60, seriously injuring one person and another.
View from the Israeli settlement of Har Gilo on Route 60, with the West Bank village of Beit Jala in the background. (Abir Sultan/Flash 90)
A week earlier, a woman in her 30s was killed and a young girl was seriously injured when two cars and a truck collided on the same road.
According to the latest figures from the Road Safety Authority, 318 people have been killed in road accidents in Israel since the beginning of 2022, a slight decrease compared to last year.
